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tris buffered saline tween 20 tbst  (Thermo Fisher)


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    Structured Review

    Thermo Fisher tris buffered saline tween 20 tbst
    Tris Buffered Saline Tween 20 Tbst, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tris+buffered+saline+tween+buffer+solution/TBS+with+Tween+(TBST)%2C+Solution%2C+Molecular+Biology+Grade%2C+Ultrapure/pm42283820-629-7-24
    Average 97 stars, based on 1 article reviews
    tris buffered saline tween 20 tbst - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Role of ATF4 in fibroblast proliferation and apoptosis induced by mechanical trauma through the miR-93-3p/Smad7 axis.
    Article Snippet: Total protein was extracted from L929 cells with radio-immunoprecipitation assay lysis buffer and phenylmethylsulfonyl fluoride (both from Servicebio Biotechnology Co., Ltd., Wuhan, Hubei, China), and the concentration of the extracted protein was analyzed by bicinchoninic acid kits (Thermo Scientific). .. Subsequently, 20 μg protein sample was detached by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred onto polyvinylidene fluoride membranes (Merck Millipore Corp., Billerica, MA, USA), which were then sealed with 5% skim milk in tris-buffered saline-tween buffer solution (Thermo Scientific). .. Afterwards, membranes were cultivated with primary antibodies ATF4 (#11815, 1:1000, Cell Signaling Technology, Danvers, MA, USA), Smad7 (ab216428, 1:500, Abcam, Cambridge, UK), proliferating cell nuclear antigen (PCNA, #13110, 1:1000, Cell Signaling Technology), Caspase−3 (#9662, 1:1000, Cell Signaling Technology), β-actin (ab213262, 1:1000, Abcam) and GAPDH (#2118, 1:1000, Cell Signaling Technology) at 4°C overnight, and then were cultivated with a secondary antibody (#7074, 1:3000, Cell Signaling Technology) for 1 h. Chemiluminescence detection was conducted with hypersensitivityenhanced chemiluminescence (Biosci Biotechnology Co, Ltd., Wuhan, Hubei, China).

    Saline:

    Article Title: Role of ATF4 in fibroblast proliferation and apoptosis induced by mechanical trauma through the miR-93-3p/Smad7 axis.
    Article Snippet: Total protein was extracted from L929 cells with radio-immunoprecipitation assay lysis buffer and phenylmethylsulfonyl fluoride (both from Servicebio Biotechnology Co., Ltd., Wuhan, Hubei, China), and the concentration of the extracted protein was analyzed by bicinchoninic acid kits (Thermo Scientific). .. Subsequently, 20 μg protein sample was detached by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred onto polyvinylidene fluoride membranes (Merck Millipore Corp., Billerica, MA, USA), which were then sealed with 5% skim milk in tris-buffered saline-tween buffer solution (Thermo Scientific). .. Afterwards, membranes were cultivated with primary antibodies ATF4 (#11815, 1:1000, Cell Signaling Technology, Danvers, MA, USA), Smad7 (ab216428, 1:500, Abcam, Cambridge, UK), proliferating cell nuclear antigen (PCNA, #13110, 1:1000, Cell Signaling Technology), Caspase−3 (#9662, 1:1000, Cell Signaling Technology), β-actin (ab213262, 1:1000, Abcam) and GAPDH (#2118, 1:1000, Cell Signaling Technology) at 4°C overnight, and then were cultivated with a secondary antibody (#7074, 1:3000, Cell Signaling Technology) for 1 h. Chemiluminescence detection was conducted with hypersensitivityenhanced chemiluminescence (Biosci Biotechnology Co, Ltd., Wuhan, Hubei, China).



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